High content screening (HCS) is a method used to analyze the effect of a particular compound on cells. In HCS, cells are grown in 96- or 384-well plates and exposed to various compound concentrations. The cells are then stained with fluorescent dyes and analyzed using a microscope. The staining allows for the identification of specific cellular features, such as the size or shape of the nucleus, that can be used to quantify the effect of the compound on the cells.